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Associate professor Research team: EcoPEPS e-mail:  jean-bernard.cliquet@unicaen.fr  Tel: +33(0)2 31 56 53 03 Location: CAMPUS 1, Building, J3, Door IR 066  Address: Université de Caen Normandie, CS 14032 Esplanade de la paix, 14032 CAEN CEDEX 5, France

Research activities

My research activities are focused on the ecophysiology of grassland species and legumes and revolve around 2 axes:

(1) Measurement of carbon and nitrogen rhizodeposition. I use stable isotopes, ¹³C and ¹⁵N, to study the determinants of the rhizodeposition of both elements, in particular via the exudation of soluble compounds. I am particularly interested in the ability of grassland legumes or field crops to fix both atmospheric carbon and nitrogen and to transfer them to the soil. This rhizodeposition contributes to the storage of C in the soil and to the N enrichment of this soil. Interactions with soil microorganisms are studied through collaborations with microbiologists. In collaboration with colleagues at Unilasalle Rouen, I am also interested in the stabilization of C from legume crop residues in soil organic matter in relation to soil mineralogical characteristics.

(2) Use of atmospheric S by plants. I use a stable isotope of S, 34S, to measure the ability of different species to use atmospheric S. This capacity of plants is studied both to assess the use of plants to use pedospheric and atmospheric sources of S, and to assess the impact of this fixation on the reduction of the levels of polluting S compounds.

Recent and current projects

• P2C « la plante : « pilote de la capture et du transfert de carbone vers le sol des prairies » 2015-2018 : Programme ADEME (REACTIFF) porté par A. Bertrand-Morvan, UMR EVA-Caen
• BEER « Bactéries, Exsudats et Rhizodépots » Projet RIN Normandie 2019-2022
• SMN »Soja Made In Normandie » Projet inclus dans la Plan Régional Normand des protéines végétales destinées à l’alimentation humaine
• Silicol « Stimuler l’association Colza-Légumineuses : vers la définition d’un idéotype de colza apte à l’association, vigoureux et productif en conduite agroécologique » Projet Promosol 2022-2024

Supervision

 PhD

• Co-supervison of Anouk Lyver (sept 2021-sept 2024) “Augmentation de l’accumulation de carbone dans les sols normands par la culture des légumineuses”
• Co-supervison of Mohamed Kante (sept 2018 - juil 2022) « Impact de la rhizodéposition du C et du N par les Fabacées à graines et les Fabacées fourragères sur les communautés microbiennes édaphiques et sur la dynamique Du C et du N dans les sols»
• Co-supervison of Caroline Kohler (2009-2011) « Contribution des espèces végétales au stockage du carbone dans les prairies de Basse-Normandie « 
• Co-supervison of Sébastien Varin (2005-2009) “Plasticité fonctionnelle du trèfle blanc en réponse à la disponibilité en soufre.”
• Supervison of Fabien lesuffleur (2033-2007) « Rhizodépostion à court terme de l’azote et exsudation racinaire des acides aminés par le trèfle blanc (Trifolium repens L.). »
• Supervision of Florence Paynel (1998-2002) « Transfert de composés azotés entre le trèfle blanc et le ray-grass anglais. Etude de l'exsudation racinaire de l'ammonium et des acides aminés »

Master 2 Research (recent years)

• Co-encadrement de P. Bergerot (2021) : « Effets des biostimulants sur la croissance et les capacités de fixation de l’azote et du carbone atmosphérique par le soja»
• Co-encadrement (50%) de M. Kanté (2018) : « Dépollution du soufre atmosphérique et de l’ammoniac par des plantes de milieu humide, mise au point d’une méthode d’étude. »
• Co-encadrement (50%) de C. Roussel (2017) : « “Analyse des capacités de transfert du carbone dans le système plante-sol dans des monolithes provenant de prairies à stock de carbone contrastés“.

Publications

• Kante M, Riah-Anglet W, Trinsoutrot-Gattin I, Cliquet JB (2023) Rhizodeposition through root senescence and root exudation of atmospheric C and N is controlled by traits indicative of resource acquisition and root development. Journal of Ecology, https://doi.org/10.1111/1365-2745.14107
• Cliquet JB, Roussel C, Lemauviel-Lavenant S, Morvan-Bertrand A, Cliquet JB (2022) Fate of recently fixed C in plant-soil monoliths from permanent grasslands. In Grassland at the heart of circular and sustainable food systems (eds. L. Delaby, R. Baumont, V. Brocard, S. Lemauviel-Lavenant, S. Plantureux, F. Vertes and J.L. Peyraud), Grassland Science in Europe, vol. 27, pp. 119-121.
• Kante M, Riah-Anglet W, Cliquet JB, Trinsoutrot-Gattin (2021) Soil enzyme activity and stoichiometry; linking soil microorganism resource requirement and legume carbon rhizodeposition. Agronomy, doi.org/10.3390/agronomy11112131
• Kante M, Lemauviel-Lavenant S, Cliquet JB (2021) Remediation of atmospheric sulfur and ammonia by wetland plants: development of a study method. Int J of Phytoremediation, doi.org/10.1080/15226514.2021.1949264
• Kohler C,  Morvan-Bertrand A, Lemauviel-Lavenant S, Gallard Y, Cliquet JB (2020) Comparaison de l’impact de deux méthodes de rénovation d’une prairie du domaine expérimental INRAE du Pin-au-Haras sur la productivité et l’évolution des stocks de Carbone du sol : le resemis et le sursemis. Fourrages, 243.
• Kohler C,  Morvan-Bertrand A,  Cliquet JB, Klumpp K Lemauviel-Lavenant S (2020) Tradeoff between the conservation of soil C stocks and vegetation productivity in temperate grasslands. Agronomy, 10,1024. doi.org/10.3390/agronomy10071024
• Tasset E, Morvan-Bertrand A, Amiaud B, Cliquet JB, Louault F, Klumpp K, Vecrin R, Mischler P, Husse S, Lemauviel-Lavenant S (2019) Des « bouquets de services écosystémiques » rendus par les prairies permanentes de fauche. Fourrages, 237:83-94.
• Cliquet JB, Lemauviel-Lavenant S (2019) Grassland species are more efficient in acquisition of S from the atmosphere when pedospheric S availability decreases. Plant Soil. 435:69-80. doi.org/10.1007/s11104-018-3872-6
• Legay N, Personeni E, Slezack-Deschaumes S, Piutti S, Cliquet JB (2014) Grassland species show similar strategies for sulphur and nitrogen acquisition. Plant Soil. 375:113–126. doi.org/10.1007/s11104-013-1949-9
• Varin S, Lemauviel-Lavenant S,  Cliquet JB  (2013) Is white clover able to switch to atmospheric sulphur sources when sulphate availability decreases? J Exp. Bot 64: 2511-2521. doi.org/10.1093/jxb/ert109
• Lesuffleur F, Salon C, Jeudy C & Cliquet JB (2013) Use of a 15N₂ labelling technique to estimate exudation by white clover and transfer to companion ryegrass of symbiotically fixed. Plant Soil 369:187–197. doi.org/10.1007/s11104-012-1562-3
• Lesuffleur F, Cliquet JB (2010)  Characterisation of root amino acid exudation in white clover. Plant Soil, 333, 191-201. doi.org/10.1007/s11104-010-0334-1
• Varin S, Cliquet JB, Personeni E, Avice JC, Lemauviel-Lavenant S (2010) How does sulfur availabilty modify N acquisition of white clover (Trifolium repens L.). J Exp. Bot., 61, 225-234. doi.org/10.1093/jxb/erp303
• Fustec J, Lesuffleur F, Mahieu S, Cliquet JB (2010) Nitrogen rhizodeposition of legumes. A review Agron. Sustain. Dev. 30, 57-66. doi.org/101051/agro/2009003
• Lee BR, Jung WJ, Avice JC, Cliquet JB, Ourry A, Kopriva S, Kim TH (2009) Increase of proline loading to phloem and its effect on nitrogen uptake and assimilation in water-stressed white clover. New Phytol. 182, 654-663.doi.org/10.1111/j.1469-8137.2009.02795.x
• Tallec T, Diquélou S, Lemauviel-Lavenant S, Cliquet JB,  Lesuffleur F, Ourry A (2009) Availability of N and S affect nutrient acquisition efficiencies differently by Trifolium repens and Lolium perenne when grown in monoculture or in mixture. Environ. Exp. Bot. 66, 309-316. doi.org/10.1016/j.envexpbot.2009.02.002
• Varin S, Leveel B, Lemauviel-Lavenant S, Cliquet JB (2009) Does the white clover response to sulphur availability correspond tophenotypic or ontogenetic plasticity? Acta Oecologica, 35, 452-457. doi.org/10.1016/j.actao.2009.01.002
• Varin S, Lemauviel-Lavenant S, Cliquet JB, Diquélou S, Michaelson-Yates TM (2009) Functional plasticity of Trifolium repens L. in response to sulphur and nitrogen availability. Plant Soil, 317, 189-200. doi.org/10.1007/s11104-008-9800-4 
• Tallec T, Diquélou S, Lemauviel-Lavenant S, Cliquet JB,  Lesuffleur F, Ourry A (2008) Nitrogen:sulphur ratio alters competition between Trifolium repens and Lolium perenne under cutting: Production and competitive abilities. Eur. J. Agronomy. 29, 94-101. doi.org/10.1016/j.eja.2008.04.004
• Limami AM, Glevarec G, Ricoult C, Cliquet JB, Planchet E (2008) Concerted modulation of alanine and glutamate metabolism in young Medicago truncatula seedlings under hypoxic stress. J Exp. Bot. 59, 2325-2335. doi.org/10.1093/jxb/ern102
• Paynel F, Lesuffleur F, Bigot J, Diquelou S, Cliquet JB (2008) A study of ¹⁵N transfer between legumes and grasses. Agron. Sustain. Dev. 28, 281-290.
• Vandenkoornhuyse P, Mahé S, Ineson P, Staddon P, Ostle N, Cliquet JB, Francez AJ, Fitter AH, Young PW (2007) Active root-inhabiting microbes identified by rapid incorporation of plant-derived carbon into RNA. P.N.A.S. 104, 16970-16975.
• Lesuffleur F, Paynel F, Bataillé MP, Le Deunff E, Cliquet JB (2007) High proportions of glycine and serine in root exudates of six different species result from high efflux rates. Plant Soil 294, 235-246.
• Ricoult C, Echeverria LO, Cliquet JB, Limami AM (2006) Characterization of alanine amino transferase (AlaAT) multigene family and hypoxic response in young seedlings of the model legume Medicago truncatula. J. Exp. Bot. 57, 3079-3089.
• Amiard V, Morvan-Bertrand A, Cliquet JB, Billard JP, Huault C, Sandström J, Prud’homme MP (2005) Carbohydrate and amino acid composition in Lolium perenne phloem sap before and after defoliation. Can. J. Bot. 82, 1594-1601.
• Ricoult C, Cliquet JB, Limami AM (2005) Stimulation of alanine amino transferase (AlaAT) gene expression and alanine accumulation in embryo axis of the model legume Medicago truncatula contribute to anoxia stress tolerance. Physiol. Plant. 123, 30-39.
• Beuve N, Rispail N, Lainé P, Cliquet JB, Ourry A, Le Deunff E (2004) Putative role of g-aminobutyric acid (GABA) as a long distance signal in up-regulation of nitrate uptake in Brassica napus L., Plant Cell Environ. 27, 1035-1045.
• Glevarec G, Bouton S, Jaspar E, Riou MT, Cliquet JB, Suzuki A, Limami AL (2004) Respective roles of GS/GOGAT and GDH in ammonium and amino acid metabolism during germination and post-germinative growth in the model legume Medicago truncatula. Planta 219, 286-297.
• Paynel F, Cliquet JB (2003) N tranfer from white clover to perennial ryegrass, via exudation of nitrogenous compounds. Agron. Sustain. Dev. 23 : 503-510.
• Paynel F, Murray PJ, Cliquet JB (2001) Root exudates : a pathway for short-term N transfer from clover and ryegrass. Plant Soil 229 : 235-243
• Faure S, Cliquet JB, Thephany G, Boucaud J (1998) Nitrogen assimilation in Lolium perenne L. infected with an arbuscular mycorrhizal fungus Glomus fasciculatum . New Phytol. 138, 411-417.
• Cliquet JB, Murray PJ, Boucaud J (1997) Effect of the arbuscular mycorrhizal fungus glomus fasciculatum on the uptake of amino nitrogen by Lolium perenne L. New Phytol. 137, 345-349.
• Murray PJ, Hatch DJ, Cliquet JB (1996) Impact of insect herbivory on the growth, nitrogen and carbon contents of white clover (Trifolium repens L.). Can. J. Bot. 74, 1591-1595
• Deleens E, Cliquet JB, Mariotti A, Prioul JL (1994) Using ¹³C and ¹⁵N plant label near natural abundance range in biochemistry. Aust. J. of Plant Physiol. 21, 133-146.
• Cliquet JB, Stewart GR (1993) Ammonia assimilation in Zea mays L. infected with a vesicular-arbuscular mycorhizal fungus Glomus fasciculatum. Plant Physiol. 101, 865-871.
• Cliquet JB, Boutin JP, Deleens E, Morot-Gaudry JF (1991) Ethephon effects on translocation and partitioning in maize. Plant Physiol. Biochem., 29, 623-630.
• Cliquet JB, Deleens E, Mariotti A (1990) C and N mobilisation from stalk and leaves during kernel filling by ¹³C and ¹⁵N tracing in Zea mays L. Plant Physiol. 94, 1547-1553.
• Cliquet JB, Deleens E, Bousser A, Martin M, Lescure JC, Prioul JL, Mariotti A, Morot-Gaudry JF (1990) Estimation of carbon and nitrogen allocation during stalk elongation by ¹³C and ¹⁵N tracing in Zea mays L. Plant Physiol. 92, 79-87.
• Cliquet JB, Morot-Gaudry JF (1989) Comparaison de la ribulose bisphosphate carboxylase de feuille et de tige de maïs. C.R. Acad. Sci. 309, III. 583-586
• Cliquet JB, Morot-Gaudry JF (1989) Effet de l'éthéphon sur la photosynthèse du maïs. C.R. Acad. Sci. 309, III. 317-332.
• Cliquet JB, Martin M, Morot-Gaudry JF (1988) Storage of C compounds and N compounds in maize stalks. Plant Physiol. Biochem., 26, 206-207