Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Institut Agro - Agrocampus Ouest

Home page

Amino acid efficiency in dairy cows

Amino acid efficiency in dairy cows
Improving amino acid efficiencies in dairy cows: partitioning between protein syntheses and AA catabolism.

Environmental, economic and protein autonomy issues

Increasing metabolisable protein efficiency for milk protein synthesis is an important environmental, economic and protein autonomy issue for dairy nutritionist.
This efficiency partly depends on the metabolic fate of AA, the nutrients actually absorbed in the small intestine after the hydrolysis of metabolisable proteins. Indeed, the absorbed AA are either used to resynthesize different proteins synthesised by the cows, mainly milk protein and metabolic faecal protein, or catabolised into urea. However, protein synthesis and turnover is a costly process in term of energy.
The aim of this work was to understand the variations in metabolisable protein and individual AA efficiencies in response to net energy supply (starchy diets or post-rumen glucose). The effects of net energy supply were studied either in interaction with two levels of MP supply (i.e. two amounts of total AA supplies) or in interaction with 2 AA profiles (Low and high supplies in lysine, methionine and histidine) in dairy cows. For this, we analysed mammary and liver metabolism.

An original approach to assess amino acid catabolism based on the INRA (2018) feeding system

The catabolism of essential AAs was assessed both by measuring portal, hepatic and mammary gland net fluxes of AA and by calculating whole body efficiency of each essential AA (EAA) using INRA (2018) : the inefficiency is an estimate of AA catabolism. The efficiency of each individual EAA was calculated from the metabolisable protein (MP) efficiency (expressed as PDI efficiency in INRA, 2018 for Protein Digestible in the Intestine) proposed in the INRA (2018) ruminant feeding system. The MP efficiency corresponds to the ratio between the sum of export proteins (milk protein yield and metabolic faecal, scurf, gestation etc.) by the animal and the digestible protein intake (Figure 1; INRA 2018). It was combined with the EAA profiles of the different protein exported (milk, metabolic faecal, scurf) to define individual EAA efficiencies.

Metabolisable protein efficiency
Figure 1. Metabolisable protein (Protein Digestible in the Intestine) efficiency according INRA (2018) feeding system (from Lemosquet and Omphalius)

Metabolisable protein and AA efficiencies in response to net energy or/and metabolisable protein supplies

The increased MP efficiency observed with increasing net energy supply was explained by an increase in milk protein yield without any increase in MP supply.
In one hand, increasing net energy supply increased mammary uptake of histidine, methionine, phenylalanine and tyrosine (group 1 AA according to Mepham, 1982), and of some non-essential AA. On the other hand, the mammary catabolism of lysine, isoleucine, leucine and valine (group 2 AA) and the hepatic catabolism of group 1 AA decreased in parallel.
Metabolisable protein efficiencies decreased with increasing MP supplies because milk protein yield and mammary uptake of AA increased to a lesser extent than AA supplies. At the same time, hepatic catabolism of AA from group I and mammary catabolism of AA from group 2 increased. These different mechanisms would explain the additivity of the observed increases in milk protein in response to increasing, both net energy and MP supplies.

Finally, the MP efficiency could rely on the most limiting EAA presenting the highest efficiency. This shows the importance of considering the efficiencies by AA and not only the MP efficiency (PDI) in a future feeding system for dairy cows.
The effects of starchy  energy diets and better balancing AA supply on nitrogen, MP and AA efficiencies will be studied (see also the PhD project of Jean-Charles Anger) at different stages of lactation in order to improve the estimates of individual EAA efficiency in feeding systems.

This project was conducted in partnership between INRAE UMR PEGASE (S. Lemosquet) and Agriculture and Agri-Food Canada (H. Lapierre) within the framework of a Cifre PHD student (C. Omphalius) with the company ADISSEO (L. Bahloul).

Bibliography

  • Omphalius, C., Lemosquet, S., Ouellet, D.R., Bahloul, L., Lapierre, H. (2020). Postruminal infusions of amino acids or glucose affect metabolisms of splanchnic, mammary, and other peripheral tissues and drive amino acid use in dairy cows. Journal of Dairy Science, 103 (3), 2233-2254. [DOI], [HAL].
  • Omphalius , C., Lapierre, H.,  Bernard, L., Bahloul, L., Lemosquet,S .(2020). Responses to methionine, lysine plus histidine and energy supply on mammary metabolism in dairy cows. Presented at: EAAP 2020 Virtual Meeting. 1st-4th December 2020. [HAL].
  • Omphalius, C. Lapierre, H., Guinard-Flament, J., Lamberton, P., Bahloul, L., Lemosquet, S. (2019). Amino acid efficiencies of utilization vary by different mechanisms in response to energy and protein supplies in dairy cows. Journal of Dairy Science, 102 (11): 9883-9901. [DOI], [HAL].
  • Omphalius, C., Lapierre, H., Bahoul, L., Lemosquet, S. (2019). Responses to methionine, lysine plus histidine at two levels of energy in dairy cows. Presented at: 6. International Symposium on Energy and Protein Metabolism (Isep), Belo Horizonte, br (2019-09-09 - 2019-09-12), [DOI], [HAL].
  • Omphalius, C., Lemosquet, S., Ouellet, D.R., Bahoul, L., Lapierre, H. (2019). Protein and energy affect splanchnic and mammary metabolisms and drive amino acid use in dairy cows. Presented at : 6. International Symposium on Energy and Protein Metabolism (Isep), Belo Horizonte, br (2019-09-09 - 2019-09-12). [DOI], [HAL].
  • Omphalius, C., Lapierre, H., Bahloul, L., Lemosquet, S. (2019). Methionine, lysine, and histidine supplementation at low and high net-energy supply in dairy cows. Presented at: Adsa Annual Meeting, Cincinnati, us (2019-06-23 - 2019-06-26). [HAL].
  • Omphalius, C., Lapierre, H., Guinard-Flament, J., Bahloul, L., Lemosquet,  S. (2018). L’efficience d’utilisation des acides aminés varie via des mécanismes différents lors d’apports contrastés d’énergie nette et de protéines digestibles dans l’intestin. Presented at: Rencontres autour des recherches sur les ruminants (3R), Paris, fr (2018-12-05 - 2018-12-06). [HAL].