Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free:

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site:, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Google Analytics

Targeted advertising cookies


The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at or by post at:

24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal


Zone de texte éditable et éditée et rééditée

Cell cryopreservation in aquatic species

Cell cryopreservation in aquatic species
© SYSAAF Pierrick Haffray
Our aim is to i) optimize and standardize cryopreservation of resources interesting for fish farmers, breeders, genetic resources managers, and researchers, according to a procedure which encompasses in field collect and conservation and cryobanking of the cryopreserved material; ii) Optimize the cellular and molecular damages induced by cryopreservation, in order to identify the leverage levels in refractory cell types or species, and to control the risks for the offspring

The uses of cryopreserved cells

Cell cryopreservation and cryobanking in aquatic species is a unique tool to preserve the genome of domesticated species over the many steps of selective breeding, the later being increasingly developed since the 1990ies, to diffuse the genetic progress, and to facilitate broodstock management by extending or delaying offspring production. To some extent, it can also provide a back-up tool in the case of endangered species.


New species, new cell types candidate for cryopreservation

In the past, most of our research was dedicated to sperm cryopreservation. Today, only few species have spermatozoa that cannot be successfully cryopreserved. In fish, oocytes and whole embryos cannot be cryopreserved because of the fragility of the yolk sac. This is why we set up the cryopreservation of fin cells and tissues which allow the preservation of both parental genome whatever the age or sex of the donor. In the same line, we are optimizing the cryopreservation of germinal stem cells, whose grafting into the gonad of a recipient fry allows the production of both spermatozoa and ova bearing the donor genome. Last, we set up a new method for oyster larvae cryopreservation.


Epigenetic risk assessment after cryopreservation

In fish, most of the most efficient cryoprotectants bear reactive methyl groups (methanol, dimethyl sulfoxide, dimethyl formamide) known to provide methyl groups to DNA in oxidative conditions. We are developing an important project to determine the stability of the DNA methylation profile after cryopreservation in several farmed species. We are also characterizing whether some specific regions in sperm chromatin are more fragile towards cryopreservation, in order to assess the risk to transmit alterations to the next generation. Indeed, it is now well recognized that the DNA methylation profile in sperm plays a role in the proper establishment of gene expression after the embryonic genome activation and during development.