MicroHippo

MicroHippo - Hippo pathway-mediated regulation of micropyle formation by microRNA 202 (miR-202) in the fish oocyte

National program coordinated by Julien Bobe (selected in 2021)

Female fish lacking miR-202 exhibit major fertility defects with low fertilization rates. Preliminary evidence have revealed defects in the formation of the micropyle, a particular structure which, during fertilization, guides sperm to the mature oocyte. Micropyle originate from a single cell of the granulosa cell layer, the micropylar precursor cell (MPC), that differentiates at the animal pole of the oocyte under the control of the Hippo pathway. The objective of the project is therefore to understand how miR-202 regulates MPC differentiation (and ultimately micropyle formation) through the modulation of the Hippo pathway. In the MicroHippo project, a molecular 3D phenotyping of MPC differentiation will be carried out in miR-202 mutants and miR-202 targets will be identified by transcriptional spatial profiling. The in vivo relevance of identified target will subsequently be validated using a genome-editing based strategy directed against miR-202 binding sites in the 3’ UTR region.

Partner

Hervé Seitz IGH Montpellier : https://www.igh.cnrs.fr/fr/recherche/departements/genetique-et-developpement/impact-systemique-des-petits-arn-regulateurs

Modification date : 28 March 2023 | Publication date : 19 August 2021 | Redactor : Agnès Girard