Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Agrocampus Ouest Rennes 1 University Logo Igepp

Home page

Characterization of Genetic Diversity

Research

Context and Issues

Plant breeding programs success largely depend on the exploitation of a large range of genetic variability mainly collected into genetic resources collections. The BrACySol Biological Resources Centre (http://florilege.arcad-project.org/fr/crb/bracysol) maintains genetic resources collections of different cultivated species, including Solanum and Brassica. The Solanum collection gathers: the cultivated species Solanum tuberosum (cultivars and hybrids), dihaploids, accessions belonging to 32 wild species and interspecific hybrids. All accessions are maintained as clones by vegetative multiplication. The Brassica collection gathers the cultivated species Brassica napus, Brassica oleracea, Brassica rapa, few accessions from B. carinata, B. nigra and B. juncea. Accessions are spread into national, network and INRAE collections.

The characterization of the genetic diversity is under progress using different types of descriptors: morphological, agronomical and technological traits, molecular data (SSR, SNP, GBS …). A better knowledge of the genetic diversity present in these collections help for instance to characterize our wide collections and deeply help to optimizing the choice of parents for mapping populations, association panels, or definition of core collections.

Objectives

  • Optimizing and securing the management of the genetic resources
  • Broadening and characterizing the genetic diversity of Brassica (both cultivated and wild) and Solanum species
  • Characterizing the phenotypic diversity of different traits of interest

Methodology

  • Clones produced annually under field, platform and in vitro (Solanum), seed production taking into account the structure of resources (populations or pure lines)
  • Genotyping
  • Phenotyping
  • Design of core collections and panels

Main Results

We optimize and secure the management of the genetic resources. The accessions are maintained either by yearly vegetative propagation for Solanum or by sexual crosses with multiplication every 10 to 15 years for Brassica. In order to limit the number of regeneration cycle, we developed cryopreservation methods from meristem for Solanum and seeds for Brassica. Molecular fingerprinting of the potato varieties and B. oleracea accessions has been performed using SSR markers for identification of each varieties and of duplicates within the collection.

We enlarge available biological resources. The Solanum collection was enriched with breeding lines and varieties improved for resistance to different pathogens as well as with the S. phureja clone that was used to develop the reference genome. For Brassica, we realized that an important wild diversity absent from our and the worldwide core collection, has to be collected and introduced (Prima BrasExplor project that started in 2020).

We characterize the diversity for different traits of interest. Solanum accessions have been evaluated for resistance to late blight disease, to cyst nematode and to Pectobacterium, in collaboration with RA team and FN3PT. A core collection was developed from the potato varieties collection. In Brassica sp., a large screening of Brassica genotypes (especially B. rapa, B.oleracea, B. napus) was performed for various biotic stresses in collaboration with RA and EGI, for warm temperature susceptibility in collaboration with Demecol team. In B. napus, the metabolomic diversity and its associated microbiota composition are assessed in collaboration with RA and RCA teams.

Partners

  • FN3PT
  • ACVNPT
  • University Houari Boumedienne Alger (USTHB)

Funding and Support (last 5 years)

  • GIS IBiSA, ‘CertBracysol’ (2016 – 2018) :Towards NF S 96-900 quality certification of the BrACySol BRC (P.I. F. Esnault)
  • PHC Tassili (2016-2019) Biologie florale et dynamique des populations diploïdes et polyploïdes (P.I. A.M. Chèvre)
  • Région Bretagne-FEDER ‘Gwassica’ : Limiter l’impact des aléas climatiques sur chou-fleur via la création variétale et le développement d’outils de phénotypage (P.I. V. Tremellat, OBS)
  • H2020-SFS-2015-2, ‘G2P-Sol’ (2016 – 2021): Linking genetic resources, genomes and phenotypes of solanaceous crops (P.I. G. Giuliano, ENEA, Italie)
  • Ecophyto, ‘PoTStaR’ (2017 – 2021) : Stabilité des résistances au mildiou de la pomme de terre et identification de marqueurs associés (P.I. F. Esnault)
  • CASDAR, ‘IdEvol’ (2019 – 2022) :  Evolution technologique pour l’identification des variétés de pomme de terre par marqueurs microsatellites en appui à la certification des plants (P.I. S. Marhadour, FN3PT)
  • Ministère Agriculture et GNIS, ‘BraSol’ (2019 – 2021) : Sécurisation et caractérisation des collections Brassica et Solanum (P.I. F. Esnault)
  • H2020 Prima ‘BrasExplor’ (2020-2023) : Wide exploration of genetic diversity in Brassica species for sustainable crop production (P.I. A.M. Chèvre)

Publications  (last 5 years)

Aissiou F., Laperche A., Falentin C., Lodé M., Deniot G., Boutet G., Régnier F., Trotoux G., Huteau V., Coriton O., Rousseau-Gueutin M., Abrous O., Chèvre A. M. & Hadj-Arab H. (2018). A novel Brassica rapa L. genetic diversity found in Algeria. Euphytica, 214(12), 241.  https://doi.org/10.1007/s10681-018-2318-9

Esnault F., Pellé R., Dantec J.P., Bérard A., Le Paslier M.-C. & Chauvin J.-E. (2016). Development of a Potato Cultivar (Solanum tuberosum L.) Core Collection, a Valuable Tool to Prospect Genetic Variation for Novel Traits. Potato Research, 59(4), 329-343.  https://doi.org/10.1007/s11540-016-9332-x